GIUSEPPE DI BELLA > PRINCIPI ATTIVI > Betacarotene > Non-genomic inhibitory signaling of beta-carotene in squamous cell carcinoma of the lungs.

 
Non-genomic inhibitory signaling of beta-carotene in squamous cell carcinoma of the lungs. di Al-Wadei HA, Schuller HM.
 
 
Data: 12/04/2011
Tipologia: Area Pazienti - Documentazione
Lingua: Inglese
Pubblicazione: Int J Oncol
Anno: 2009
Fonte: Int J Oncol. 2009 Apr;34(4):1093-8
 
 
Descrizione:

Studies have suggested that retinoids prevent lung cancer by interacting with nuclear retinoid receptors. However, clinical trials with beta-carotene increased lung cancer mortality. We recently showed that beta-carotene stimulates the proliferation of small airway-derived adenocarcinoma by increasing cAMP signaling. Here, we have tested the hypothesis that beta-carotene may stimulate squamous cell carcinoma cells via similar mechanisms. We determined the effects of beta-carotene in cell lines from squamous cell carcinomas and large airway epithelia on proliferation by MTT assays in the presence and absence of inhibitors. Signaling via cAMP/PKA was measured by immunoassays and PKA activation assay. Phosphorylated ERK1/2 was determined by Western blotting. beta-carotene significantly inhibited proliferation and phosphorylation of ERK1/2 by Galphas-mediated signaling involving adenylyl cyclase, cAMP, PKA and ERK1/2. These findings introduce a non-genomic inhibitory mechanism of beta-carotene and emphasize the need for the development of marker-guided lung cancer prevention.

 
 
 

 

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